HLA Antibody Testing

Unique Aspects of Laboratory Testing

  • Comprehensive antibody screening using purified HLA molecules is available on multiple platforms (ELISA, flow cytometry, Luminex)
  • Microarrays with single HLA molecules provide the most sensitive and specific assays available
  • Epitope prediction by correlation with protein sequences is available

Tests

SOLID PHASE METHODS

  • HLA Class I PRA
  • HLA Class II PRA
  • Antibody Specificity Single Antigen ID, Class I
  • Antibody Specificity Single Antigen ID, Class II
  • HLA Class I Antibody Analysis with Titer
  • HLA Class II Antibody Analysis with Titer
  • ELISA Antibody Screen

CYTOTOXICITY

  • Quick Screen PRA T lymphocytes

Indications

SOLID PHASE METHODS

  • To detect HLA antibodies in recipients for selection of HLA mismatched donors
  • To identify compatible HLA types for transfusions
  • To detect HLA antibodies after transplantation
  • To detect IgG antibodies

CYTOTOXICITY

  • To detect antibodies against T lymphocytes
  • To detect IgM and IgG cytotoxic antibodies

Methods

SOLID PHASE METHODS

  • For bead array methods, purified HLA molecules are attached to microparticles. Binding of antibodies is detected by a flow cytometer or Luminex instrument.
  • For ELISA methods, purified HLA molecules are attached to wells. Binding of antibodies is detected by ELISA methods.
  • Purified HLA molecules consist of one of the following:
    (1) Mixtures of HLA molecules from many donors
    (2) HLA molecules from single donors
    (3) Single HLA molecules (single antigens)

CYTOTOXICITY

  • Microcytotoxicity with anti human globulin to enhance sensitivity

Turn Around Time (arrival before 10am)

SOLID PHASE METHODS

Routine test results in 5-15 business days

STAT testing in 3 business days

CYTOTOXICITY

Routine test results in 3-5 business days

STAT testing in 2 business days

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