HLA Typing

High Resolution HLA typing 

Tests

• HLA comprehensive high resolution typing (NGS typing of HLA-A,B,C,DRB1,DRB3/4/5, DQA1, DQB1, DPA1, and DPB1 loci)
• HLA-A high resolution
• HLA-B high resolution
• HLA-C high resolution
• HLA-DRB1 high resolution
• HLA-DRB3/4/5 high resolution
• HLA-DQA1 high resolution
• HLA-DQB1 high resolution
• HLA-DPA1 high resolution
• HLA-DPB1 high resolution

Purpose

• To determine HLA matching between patient and donor for hematopoietic stem cell transplantation.
• To determine sequence-level HLA matching between patient and donor for solid organ transplantation.
• To identify specific HLA types required for vaccine and immunotherapy.
• To diagnose certain HLA-associated diseases and drug-induced hypersensitivity.

Highlights  

• Next-generation sequencing (NGS) is used to determine the whole HLA gene sequences including all exons and introns.
• Comprehensive NGS typing includes all eleven major histocompatibility HLA genes: HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1.
• Every sample is concurrently typed by rSSO for all 11 HLA loci to corroborate NGS HLA types and to confirm homozygosity.
• HLA typing results are reported up to three-field format (e.g., HLA-A*01:01:01).
• Comprehensive patient and donor typing report identifies the matched donor allele at each locus as well as total loci.
• Typing report identifies mismatches at two field levels per locus with Graft-versus-Host (GvH) or Host-versus-Graft (Rejection) directions.
• Typing report includes T cell epitope (TCE) mismatches at HLA-DPB1 locus.
• Serological equivalents are provided in the NGS typing report to facilitate the interpretation of HLA allotype and donor-specific HLA antibodies.
• Expert consultation is available for all donor searches and selection.

• Routine testing reported in 3-10 business days.
• STAT testing reported in ≤ 3 business days. 

Intermediate resolution HLA typing 

Tests

• HLA class I (HLA-A, B, C) intermediate resolution typing   
• HLA class II (HLA-DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1) intermediate resolution typing 

Purpose

• To determine HLA types of solid organ transplant recipient and donor candidates.
• To evaluate living and deceased donors for solid organ transplants. 
• To screen unrelated donors for hematopoietic stem cell transplantation.
• To screen for certain HLA-associated diseases.

Highlights  

• Minimal ambiguity is achieved by using Luminex-based high definition rSSO bead assay to type all 11 HLA loci.
• A high throughput liquid handling system and a 96-well reaction plate is used to minimize inter-assay variations.
• The intermediate resolution typing report lists:

1. 1. NMDP ambiguity codes and their corresponding translations.
   2. DNA typing and serological equivalents to facilitate the interpretation HLA allotypes and donor-specific HLA antibodies. 
   3. The probability of finding a zero HLA-ABDR mismatched donor offer from UNOS within a year.

• Comprehensive patient and donor typing report identifies the mismatched donor antigen at each locus as well as for total loci.
• Deceased donors are concurrently typed by rSSO and 384-well formatted real-time PCR methods for all 11 HLA loci to produce accurate and timely results.

• Routine testing reported in 2-5 business days.
• STAT testing reported in ≤ 3 business days.
• Deceased donor typing is reported within 5 hours.